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Category, Microbiology Laboratory. Test, Test Method Description. Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method. TEST METHODS ARE DELIVERED AS A LINK IN YOUR EMAILED RECEIPT. AATCC TM, Antibacterial Activity Assessment of Textile Materials. Current individual AATCC test methods and other standards for textiles are Fabrics, Assessment of Textile Materials: Parallel Streak Method, Test Method

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AATCC Test Method

All OSHA standards and rules must also be consulted and followed. In addition, the electronic file may not be distributed hest over computer networks or otherwise. If no other bacterial species is specified, Staphylococcus aureus may be used as a representative Gram positive organism. Treated materials should be compared to an untreated corresponding material and a material specimen with known bacteriostatic activity. These safety precautions are for information purposes only.

The size of the zone of inhibition and the narrowing of the streaks caused by the presence of the antibacterial agent permit an estimate of the residual antibacterial activity after multiple washings.

Rectangular specimens cut 25 – 50 mm They can be any convenient size. Your comments are invited either for revision of this test method or for additional methods and should be addressed to the AATCC Technical Center.

The Parallel Tst Method has proven effective over a number of years of use in providing evidence of antibacterial activity against aatcc Gram positive and Gram negative bacteria.


Purpose and Scope cluding corresponding untreated controls of the same material, are placed in intimate contact with nutrient agar see 7. AATCC Test Method Limitations The method is not suitable for materials which are not migratory and are unable to diffuse thru the bacterial growth medium.

Other organisms can also be used depending on the intended end-use. Plug and sterilize at kPa 15 psi for 15 minutes. The method is useful for obtaining a rough estimate of activity in that the growth of the inoculum organism decreases from one end of each streak to the other and from one streak 174 the next resulting in increasing degrees of sensitivity.

If you feel that your comments have not received a fair hearing, you should make your views known to Christopher S. After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of tst specimen. AATCC MethodAntibacterial Finishes on Textile Materials, Assessment of, is a quantitative procedure which is adequately sensitive but is cumbersome and time consuming for meethod quality control and screening tests.

AATCC grants you a license as follows: Mix well using appropriate agitation. Therefore, when the intent is to demonstrate bacteriostatic activity by the diffusion of the antibacterial agent through agar, Method fulfills this need. This may be accomplished more easily by pressing the specimen to the agar surface with a biological section lifter or with a spatula which has been sterilized by flaming and then air cooled immediately before use.


Wear safety glasses in all laboratory areas. Therefore, every necessary and 5.

AATCC Antimicrobial Testing Lab – Accugen Laboratories, Inc.

417 comments will receive careful consideration at a meeting of the responsible technical committee, which you methoe attend. AATCC takes no position respecting the validity of any patent rights asserted in connection with any item mentioned in this test method. Department tesr Health and Human services publication Biosafety in Microbiological and Biomedical Laboratories should be consulted see The criterion for passing the test must be agreed upon by the interested parties.

Many standard textile finishing chemicals, especially crease resistant and permanent press reagents, will often give strong antibacterial activity even after many washes. To constitute acceptable antibacterial activity, there must be no bacterial colonies directly under the sample in the contact area. Avoid contamination by using good sterile technique in plating and transferring.

This test method is not for resale. Maintenance of Culture of Test Organisms 8. Neither the electronic file nor the hard copy print may be reproduced in any way.

Antimicrobial – Parallel Streak Method AATCC 147

Allow agar to gel firmly before inoculating. Uses and Aqtcc 1. At the conclusion of two weeks, make a fresh transplant from stock culture.